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In order to construct a Leslie matrix model, it is extremely important to obtain accurate data on age-speci c survival and reproductive rates for animals under various exposure conditions In many previous studies of the impacts of contaminants on sh populations, the effects of single compounds based on water column toxicity have been used to estimate survival and reproductive impacts Landahl et al (1997) note that this method requires the extrapolation from tested species of sh to untested ones, and therefore, for more realistic model development it is preferable to base estimates of population-level toxicant impacts on data collected for the species of interest As suggested above, the early study by Longwell and Hughes (1980) found changes as a result of pollution in reproduction and success of reproductive elements, but could not link these to changes at the population level Landahl et al.

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(1997) looked at approaches for determining effects of pollution on sh populations of Puget Sound (USA) The primary objectives of the study were to compare survival rates, reproduction rates and projected population growth rates of English sole (Parophrys (Pleuronectes) vetulus) from sites in Puget Sound with different levels of sediment contamination (Landahl & Johnson, 1993; Johnson & Landahl, 1994) Using eld and laboratory data, they determined vital rates and other life history parameters in English sole subpopulations from urban and non-urban sites in Puget Sound, and used this information to estimate potential population level impacts of anthropogenic stressors, with a generalised Leslie matrix model Landahl et al (1997) found that initial model projections indicated that contaminant effects, particularly in relation to reproductive capacity, could substantially reduce the intrinsic rate of increase (r) of English sole populations from contaminated sites in Puget Sound.

Additionally, estimated reductions in r were comparable to reductions in r associated with shing mortality rates between 15% and 30%, suggesting that contaminant impacts are a cause for concern However, although the initial model provides insight into the potential effects of chemical contaminants on English sole populations, Landahl et al (1997) note that several re nements are required in order to increase the model s ecological relevance Firstly, better estimates of age-0 to age-3 survival obtained from eld and laboratory studies with larval and juvenile English sole are critical as the model is extremely sensitive to these parameters In addition, improved data is needed on both shing and natural mortality for Puget Sound Secondly, the model could be strengthened by a more.

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Cyclic voltammetry is one of the most useful techniques for studying chemistry in non-aqueous solutions. It is especially useful in studying electrode reactions that involve an unstable intermediate or product. By analyzing cyclic voltammograms, we can elucidate the reaction mechanisms and can determine the thermodynamic and kinetic properties of the unstable species. Some applications were described in previous sections. Much literature is available concerning cyclic voltammetry dealing with the theories and practical methods of measurement and data analysis [66]. In this section, three useful cyclic voltammetry techniques are outlined.

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Because the ions in electrolyte solutions are often more or less associated, Eq. (7.5) is useful in analyzing conductivity data. The experimental data for K and c are subjected to computer analysis, by applying the least-squares method, and optimum values of such parameters as KI , KA and a are obtained. Sometimes the ion parameter a (i.e. the distance of closest approach) is replaced by the Bjerrum s distance q in Section 2.6. In this case, the parameters obtained from Eq. (7.5) are of two kinds, KI and KA.

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